D. ananassae fosmid 2594O08 - Kelly Hutchins

olf413 sequence Gene Record Finder. Gives the 10 exon pieces.

>olf413:2_701_0
MAHPRKAVATPATLQLGPPAQTAQSPAATLRHSRTASSSRRLSLIRCFIS
CHTFNLFLLLLLLASGVRAASKLATRSNKTSGSSTASGVGAGTAATSAAA
AAASGTPIWDHAIDLNDDFRILWQIINQDITFEIQARTLGYVGFGFSPDG
NLAGADMAIGWVDKGQTYFQ


>olf413:3_701_0
DRHVTRNGDPEPVVDPSQDYMLMLGYENATHTVLRFRRKLDTCDPSHDIA
IT


>olf413:4_701_0
NDTMRLLYMYHAQDPPHGSVRPGTLPDPARAFRPYRPMVLMQRAQLPMPS
PTHDERVRVLELRNEDVELPAGDLPLFWCKMFKLEDINRKHHLIR


>olf413:5_701_0
YEPIYDSSSSVHYLQHITLHECQGAHAELEEMAREQGRPCLGARSIPLAC
NAIVASWSRGSE


>olf413:6_701_0
GFTYPHEAGYPIESRQAKYYLMETHYNNLKPDFAQLHARQMADNSGLKIY
FTHVLRPNDAGTLSI


>olf413:7_701_2
MDPNWRHIIPPGQKRVVSEGQCIEDCTGYAFPQQGINIFAVMMRTHQIGK
EVKLRQ


>olf413:8_701_0
IRQTEELPPIAHDSNIDVAYQDFRRLPQSVHSMPGDRLIAECIYDSSSRK
AITL


>olf413:9_701_2
GLTMKEESCTVLTLYYPRQKKLTTCHSLPSLPTVLHSLGIEQLA


>olf413:10_701_1
DSNPVLISSPPELAGMTLEARLISYDWENQFGEFQEATRKGSFKPICWGA
KNHVVP


>olf413:12_701_0
GSEFLEGYSINVTKTYKKHRRCKPKRPLAPPTERTAPPPASDLSELPVLH
ELDNNNIIEGAARSSRSSATDVHSLSRGSGRHFISCLLWLGASSWWLLLM
LRT*

2 of them are low E value and on opposite strand. This could be due to that I do not have the entire gene on my fosmid and these exons are on a different strand that may start the gene somewhere else.

Drive alignment is the table produced by the gene browser and zooming in to the exact positions and finding splice sites and TopHat data to confirm base length and frame alignment.

coordinates

Here is the confirmed data from the Drive table above in the Gene Model Checker. This shows that these exons are confirmed. Also shows that my gene is lacking 5' start to the olf413 gene but ends in my fosmid.

GMC checklist

Here is the Dot Plot based on the Model provided in the checker.

GMC dotplot

alignment

UCSC