Gene Finding on D. birmipes contig 29

Peter Julnes - June 18, 2013

My file [contig29.fasta] containing my Drosophila biarmipes contig was used in a blastx search of nrp's against D. melanogaster's translated nucleotide database at the National Library of Medicine.

BLASTX graphic

The visual alignment map suggested that one gene [Asator] was present in many isoforms on this particular stretch of the D. biarmipes DNA sequence.

BLASTX descriptions

A list of significant findings displayed a few results that may have been significant (Mainly Casein Kinase I and Gilgamesh) but they span the same region as the definite Asator hits.

A useful table -- Primary Asator Hit

Asator coordinates

Comparisons of the table and the visual alignments reveal that a section [895-944] is insignificant yet it gets flagged by every isoform except one. Further investigation may reveal more information about this specific segment of DNA.

GENSCAN

Genscan predicted Asator, Casein Kinase I, and a junk model sequence. Other predicted sequences were followed until they provided junk blastP's.

GENSCAN

GENSCAN

GENSCAN

GENSCAN

It appears that this contig only contains the Asator gene and its many isoforms.

---alignments for info only [Iso-PD] ----

FlyBase Asator