In general, the best preparation for the exam is to review the previous exams. Please see the exam keys with explanations for:
When reviewing the exams:
Make sure that you know how to solve the following kinds of problems:
In addition, the exam covers the following lectures:
Here are some important highlights from these lectures, presented as questions.
Q1. How do I make cDNA from mRNA?
A1. Please see the lecture notes.
Q2. How does dideoxy (Sanger) DNA sequencing work?
A2. Please see the lecture notes.
Q3. What is a restriction fragment length polymorphism (RFLP)?
A3. Please see the lecture notes.
Q4. How do I make a targeted mutation (knockout) in a mouse?
A4. Please see the lecture notes.
Q5. What kinds of sequences are present in the human genome, and what is their relative abundance?
A5. Please see the lecture notes.
Q6. What is linkage disequilibrium?
A6. Please see the lecture notes.
Q7. What is a genome-wide association study?
A7. Please see the lecture notes.
Q8. In the ENCODE project, what kinds of sequences are associated with DNase I hypersensitivity?
A8. Please see the lecture notes, as well as these lecture notes.
Q9. In the ENCODE project, what do we learn from RNA-seq?
A9. Please see the lecture notes.
Q10. In the ENCODE project, what do we learn from ChIP-seq?
A10. Please see the lecture notes.